Tuesday, February 17, 2015

The Right Solubility Screening Kit

By Beryl Dalton


The undesirable protein aggregation restricts the form of availability which leads to limited use. Solubility screening is the best approach in establishing the best conditions or formulation that will ensure that your protein is available. Testing gives a detailed report about your protein sample. The details of this report depend on your choice of kit.

Some of the uses for the kit include quick aggregation behavior based on PH, salt and the presence of additives. It also assesses numerous conditions in a label free and parallel form. The processes require simple mixing and spinning without the use of centrifuge. This simplifies your tasks and allows you to get desired results in whichever condition.

The testing kit enables you to rescue protein samples that are in reversible form. This form of testing prevents errors that occur when the samples aggregate. These proteins are therefore available for multiple tests. The kit comes with a detailed dashboard that make interpretation of results easier because it gives a detailed trend that is easy to relate with what is in the market.

Protein aggregation hinders availability of proteins for certain functions and is therefore undesirable. The main causes of aggregation include prolonged storage, vigorous stirring, elevated temperatures and the presence of ligands or protein binding partners. The best way to avoid aggregation is to make the right decision on PH, stabilization additives and salt.

With a PH range between 3 and 10, the screening kit offer sufficient buffer. It also comes with solubility enhancers such as sugars, salts, amino acids, reducing agents and polyols, among others. The results are clear and precise which allows you to identify aggregating and non-aggregating proteins.

A single label free experiment can be used to explain over ninety formulations. The control tests are six with yes and no possibilities. A basic test is where soluble proteins go through the filters while insoluble proteins do not. This is a clear indication of the properties of these proteins that can be used to draw inferences and make conclusions.

Solubilizing proteins will give an indication of the conditions that will lead to aggregation and those that will not. The presence of proteins during filtration indicates availability in soluble form. When proteins cannot be traced during the test, it indicates availability in insoluble form.

The choice of conditions is determined by the results of screening. Since you can identify the conditions that facilitate the right behavior and the proteins in question, you can make an informed decision. It is used to confirm if the target substances are right for the expected results.

Testing kits are beneficial compared to the other methods available in the industry. They give clear results that can be interpreted based on the data available in the protein field. It is fast and convenient taking an hour to get desired results. Those results are reliable compared to the trial and error approach by other methods.

The experiment does not consume large amounts of proteins and is therefore ideal for large scale testing. Reduction in protein use allows it to be matched with assay agents. A detailed report allows more conclusions and inferences to be drawn. There is no restriction on the assay that can be used.




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